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Multi Sciences (Lianke) Biotech Co Ltd
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Cellular Technology Ltd
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Revvity
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Chem Impex International
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Chem Impex International
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Chem Impex International
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Bio-Rad
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Encapsula NanoSciences LLC
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GE Baker Hughes
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RHK Technology
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BioLynx Inc
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CHEMPUR Feinchemikalien und Forschungsbedarf GmbH
aqueous acidic solution (ph 1/4 2 controlled by 35–38% hcl, chempur) ![]() Aqueous Acidic Solution (Ph 1/4 2 Controlled By 35–38% Hcl, Chempur), supplied by CHEMPUR Feinchemikalien und Forschungsbedarf GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/control+solution/aqueous+acidic+solution++ph+1+4+2+controlled+by+35+38++hcl++chempur+/10__1039_slash_c6ra27671g-38-38-47 Average 90 stars, based on 1 article reviews
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Image Search Results
Journal: Cancer Immunology, Immunotherapy : CII
Article Title: LincRNA-p21 knockdown reversed tumor-associated macrophages function by promoting MDM2 to antagonize* p53 activation and alleviate breast cancer development
doi: 10.1007/s00262-020-02511-0
Figure Lengend Snippet: LincRNA-p21 down-regulated TAMs promoted tumor cells apoptosis, inhibited their migration and invasion. a, e LincRNA-p21 down-regulated TAMs promoted tumor cell apoptosis. 4T1 and LLC were co-cultured with lincRNA-p21 down-regulated macrophages for 48 h, respectively. The tumor cells were collected and stained with Annexin-V and 7AAD to detect the apoptosis using flow cytometry, respectively. b LincRNA-p21 down-regulated TAMs inhibited 4T1 cell proliferation. The proliferation of 4T1 cells co-cultured with lincRNA-p21 down-regulated macrophages was measured using Cell Counting Kit-8 (CCK-8) at indicated point. c, f LincRNA-p21 down-regulated TAMs inhibited tumor cells migration. Transwell assays were performed to detect migration ability of tumor cells (4T1 and LLC) co-cultured with lincRNA-p21 down-regulated TAMs for 24 h. The left panels show representative images and right panels show statistical analysis. d LincRNA-p21 down-regulated TAMs inhibited 4T1 cells invasion. Wound-healing assay was used to detect the invasive ability of 4T1 cells co-cultured with lincRNA-p21 down-regulated TAMs. g LincRNA-p21 knockdown could not increase ROS production in TAMs. The left panels show representative images and right panels show statistical analysis. h The expression of Fas on 4T1 cell and FasL on TAMs was assessed by flow cytometry. i R7050 (10 nM) was used to pretreat 4T1 for 6 h and after that 4T1 was co-cultured with lincRNA-p21 down-regulated macrophages for 48 h; 4T1 cells were collected and stained with Annexin V and PI to detect apoptosis. All the data were obtained from three independent experiments and the representative images are shown. *P<0.05 relative to control. N.S. means no significance. LLC and SLP1 mean Lewis lung carcinoma cells and lincRNA-p21 downregulated macrophages
Article Snippet: For cellular apoptosis assay, cells were stained with Annexin-V-APC and PI using the
Techniques: Migration, Cell Culture, Staining, Flow Cytometry, Cell Counting, CCK-8 Assay, Wound Healing Assay, Knockdown, Expressing, Control